Plasmid

Part:BBa_K3179103

Designed by: Jiaxian Xu   Group: iGEM19_SYSU-CHINA   (2019-10-16)


pUbC-rtTA3

pUbC-rtTA3


Usage and Biology

This part consists of promoter pUbC and basic part rtA3. pUbC from human ubiquitin C gene and is a mammalian promoter. pUbC is usually used for regular expression and has a lower expression. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.
In our experiment, pUbC-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 999
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 203
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 999
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 999
    Illegal NgoMIV site found at 473
    Illegal AgeI site found at 209
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None